|
KMID : 0364819910290040203
|
|
Korean Journal of Microbiology
1991 Volume.29 No. 4 p.203 ~ p.208
|
|
|
Molecular Cloning and Expression of dapA, the Gene for Dihydrodipicolinate Synthetase of Corynebacterium glutamicum
|
|
Oh, Jong-Won/오종원
Han, Jong-Kwon/Lee, Hyune-Hwan/Hyun, Hyung-Hwan/Lee, Jae-Heung/Chung, Stephen/한종권/이현환/현형환/이재홍/스테판정
|
|
|
Abstract
|
|
|
1
The dapA-complementing gene (L-2, 3-dihydrodipicolinate synthetase: DHDP synthetase, dapA) has been cloned by using a cosmid genomic bank of Corynebacterium glutamicum JS231 that is a lysine overproducer, AEC (s-(2-aminoethyl)-1.-cysteine) resistant mutant. By enzymatic deletion analysis, the DNA region complementing the Escherichia coil dapA host could be confined to 4.5 kb Sail-generated DNA fragment. This DNA fragment was inserted into the C glutamicum/E. coil shuttle vector pECCG117 to construct pDHDP5812. The specific activity of DHDP synthetase detected in C glutamicum JS231/pDHDP5812 was increased about 10 fold above that of C. glutamicum JS231. The addition of leucine during growth Aid not repress the expressin of dapA, and the enzyme activity was not inhibited by lysine.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|